http://localhost:8080/xmlui/handle/123456789/15 2026-04-24T16:44:16Z 2026-04-24T16:44:16Z RAHMAN, MOHAMMED LUTFUR http://localhost:8080/xmlui/handle/123456789/296 2025-01-30T06:02:59Z 2014-12-01T00:00:00Z

ISOLATION, ID ENTIFICATION AND ANTIBIOGRAM STUDY OF ESCHERICHIA COLI FROM NATIVE CHICKEN AT SYLHET REGION RAHMAN, MOHAMMED LUTFUR The study was conducted to isolate and identify Escherichia coli occurring naturally in healthy chickens. The study was also focused on the investigation of drug sensitivity and resistance pattern of the isolates. A total number of 50 field samples (cloacal swab) were collected from apparently healthy native chickens from different areas of the Sylhet region. 10 samples from Bandar Bazar, 10 samples from Ambarkhana Bazar, 10 samples from Eidgah Bazar, 10 samples from Baluchor Bazar and 10 samples from University residencial area. Out of these 50 samples of apparently healthy chicken the number of samples found to be positive for Escherichia coli were 4 of Bandar Bazar samples , 4 of Ambarkhana Bazar samples , 3 of Eidgah Bazar samples , 3 of University residencial area samples and 2 of Baluchor Bazar samples . This study reveals that the prevalence rate is 40% of Bandar Bazar , 40% of Ambarkhana Bazar ,30% of Eidgah Bazar , 30% of University residencial area and 20% of Baluchor Bazar . All of the Escherichia coli isolates revealed the same morphological ,cultural and biochemical characteristics. The antibiotic sensitivity pattern showed that among the Escherichia coli isolates (16 samples) highly sensitive to ceftriaxone, intermediate sensitive to nalidixic acid, cephalexin, gentamycin, erythromycin and chloramphenicle and fully resistant to penicillin and bacitracin. It may be concluded that the high resistance of Escherichia coli to antibiotic may constitutes a threat to public health in Bangladesh.

2014-12-01T00:00:00Z Sharmin, Karar Nusrat http://localhost:8080/xmlui/handle/123456789/295 2025-01-30T05:53:32Z 2014-12-01T00:00:00Z

SERO-SURVEILLANCE AND VACCINE EFFICACYOF PESTE DES PETITS RUMINANTS (PPR) IN SELECTED AREAS OF BANGLADESH Sharmin, Karar Nusrat The sero-surveillance and vaccine efficacy studies on Peste des Petits Ruminants (PPR) in Black Bengal goats were carried out in the district of Manikgonj during the period from January to September 2014. A total of 160 household farmers from Pouly (treatment village) and Chamta (control village) were interviewed in this study. Sero-surveillance of PPR by using competitive enzyme linked immunosorbent assay (c-ELISA) was conducted on 230 goats which were selected randomly with history of no vaccination earlier against any diseases. The per household goat population was 3.22 and 3.45 in Pouly and Chamta, respectively. Marginal farmer category was highest about 86% and 78.33% in Pouly and Chamta, respectively. Most of the farmers used tin as building material. During the study period 82% in Pouly villagers reared their animals in confined system whereas in Chamta 61.66% farmers reared their animals in free grazing system. In Pouly village only 3% farmers vaccinate their animals. In Chamta village farmers never vaccinate their animals. In Pouly 87% farmers and in Chamta 76.66% farmers preferred to treat their animals by quack. In both villages about 13% farmers treat their animals by self-treatment. In Pouly village 10.20%, 89.47% and 81.63% sero-positive cases were found pre-vaccination, 21 days post vaccination and 180 days post vaccination, respectively. In Chamta village 2.17% sero-positive cases were found in unvaccinated group and 2.08% sero-positive cases were found after 180 days.. Average sero-surveillance was 60.43% and 2.13% in Pouly and Chamta, respectively. Due to vaccination antibody level was high in Pouly but without vaccination it was low in Chamta. Thus proved that PPR vaccine working well in Pouly (treatment village).

2014-12-01T00:00:00Z Reg. No: 0235 http://localhost:8080/xmlui/handle/123456789/294 2025-01-30T05:40:58Z 2014-12-01T00:00:00Z

COMPARATIVE EFFICACY OF LENTOGENIC NEWCASTLE DISEASE VACCINES IN BROILER CHICKEN Reg. No: 0235 Newcastle disease is a devastating poultry disease all over the world including Bangladesh. The present study was conducted to investigate the comparative efficacy of ND lentogenic vaccines Cevac New L ® (Lasota strain), Avinew® (VG/GA strain) and BCRDV(F strain) in Broiler chicken during the period from January, 2014 to June, 2014 as well as isolation of Newcastle disease virus from clinical sample. For the purposes of this experiment 100 Broiler chicken were purchased from CP limited of Sylhet without history of vaccination against ND. The chickens were divided into 4 groups. In group A (n=25) and B (n=25), group C (n=25) vaccination was performed twice with BCRDV, Avinew® and Cevac New L at day 7 and 21 of age i/o, while group D (n=25) remained unvaccinated control. Serum antibody titre was evaluated by the means of hemagglutination inhibition test (HI) at 3 ® and day (pre vaccination), 14 rd th day (post vaccination ) of age. It was observed that sera sample from selected birds revealed Mean±SD of HI titre 15.36±1.79, 89.60±5.70, 215.04±20.02, after 3 th day (post vaccination) and 28 days respectively for Group A and sera sample from selected birds revealed Mean±SD of HI titre 16.96±1.79, 97.28±5.70, 271.36±20.02 after 3 rd, th th 14 and 28 rd th days respectively for Group B. It was also observed that sera sample from selected birds revealed Mean±SD of HI titre 17.60±1.79, 101.12±5.70, th ,14 and 28 rd th 271.36±20.02 after 3 days respectively.The unvaccinated control Group D maintained a Mean±SD of HI test of 0.0±0 after 28 th ,14 and 28 day of post vaccination. This indicated that there were no Ab titres to protect against NDV (F strain) in respect of HI Ab titres response. For Isolation of virus, a total of 25 tracheal, lungs, proventriculus, and brain tissue were collected from Broiler chickens where chickens dying of classical respiratory, enteric and Nervous syndrome. The samples were inoculated to embryonated chicken egg via allantoic cavity route. The positive result were death of embryo ,HA(+), HI(+)for NDV. th

2014-12-01T00:00:00Z UDDIN, A.S.M. ASHAB http://localhost:8080/xmlui/handle/123456789/293 2025-01-30T05:33:56Z 2014-12-01T00:00:00Z

ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF Escherichia coli AND Shigella spp. FROM FECAL SAMPLE OF TIGER AND LION AT DHAKA ZOO UDDIN, A.S.M. ASHAB Escherichia coli and Shigella spp. isolated from fecal samples of tiger and lion collected from Dhaka Zoo and samples were identified in the Bacteriology Laboratory of Bangladesh Livestock Research Institute,. Savar, Dhaka during the period from July to December 2014. A total of 40 samples, 18 from Tiger and 22 from Lion fecal samples were collected aseptically and subjected to primary isolation by propagating in nutrient broth followed by culture on different agar media and both. Gram’s staining and hanging drop techniques were also performed. Biochemical properties of the isolates were studied. Author found that 65% samples were E. coli positive. On the other hand 35% samples were revealed as Shigella containing samples. All the E. coli isolates were found to produce bright pink colonies on MacConkey agar, yellowish green colonies surrounded by an intense yellow green zone on Brilliant Green (BG) agar and characteristic metallic sheen colonies on the Eosine Methyline Blue (EMB) agar. In Gram’s staining technique, all the isolates were pink coloured, indicating Gram negative small bacilli while in the hanging drop technique the organisms were motile. All the E. coli isolates fermented dextrose, maltose, lactose, sucrose and mannitol with the production of both acid and gas. The results of catalase, MR and indole tests with E. coli isolates were positive but V-P test was negative. All the isolated Shigella spp. produced colorless colonies on MacConkey agar, SS agar and EMB agar. During biochemical test all the Shigella isolates ferment dextrose and lactose quickly and sucrose slowly by produced only acid but gas. In Gram’s staining showed pink color medium size, rod shaped organism and non motile in hanging drop slide. In antibiotic sensitivity test, most of the E. coli isolates were sensitive to Ciprofloxacin (CIP), Ceftriaxone (CRO), Azithromycin (AZM) and Cefotaxime (CTX) and resistant to Oxytetracyclin (OT), Erythromycin (E), Amoxicillin/Clavulanic acid (AMC) and Cephradin (CE). Isolated Shigella spp. were revealed sensitive to Ciprofloxacin (CIP), Ceftriaxone (CRO), intermediately sensitive to Amoxicillin/Clavulanic acid (AMC) and resistant to Oxytetracyclin (OT), Erythromycin (E), Azithromycin (AZM) and Cephradin (CE).

2014-12-01T00:00:00Z