http://localhost:8080/xmlui/handle/123456789/28 2026-04-16T02:35:11Z 2026-04-16T02:35:11Z RAHAT UZ ZAMAN, MD. http://localhost:8080/xmlui/handle/123456789/356 2025-03-02T05:44:23Z 2015-06-01T00:00:00Z

IN VITRO PLANT REGENERATION OF TOMATO (Lycopersicon esculentum) RAHAT UZ ZAMAN, MD. The experiment was carried out at the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet to develop a suitable protocol for high frequency plant regeneration of tomato (Lycopersicon esculentum). An efficient system for high frequency plant regeneration system of L. esculentum was developed through investigating various factors such as combination of plant growth regulators; explant type, age and ethylene biosynthesis inhibitor AgNO3. To observe the suitable medium for callus initiation and shoot regeneration, cotyledonary and hypocotyl explants of L. esculentum cv. BARI Tomato 5 were cultured on MS medium supplemented with different concentrations of 6-benzyl amino purine (BA) and α-naphthalene acetic acid (NAA). The ranges of callus initiation and shoot regeneration frequency of cotyledonary explants were 52.78% to 94.44% and 22.22% to 61.11%, respectively, whereas hypocotyl explants showed 61.11% to 97.22% and 19.44% to 58.33% callus initiation and shoot regeneration frequency, respectively. The highest callus initiation (94.44%) and shoot regeneration (61.11%) frequency were observed on MS medium supplemented with 3.0 mg/L BA and 0.2 mg/L NAA in case of cotyledonary explants. To observe the effect of age of explants source material on shoot regeneration 5 to 9 days old cotyledonary explants were cultured on MS medium supplemented with 3.0 mg/L BA and 0.2 mg/L NAA and 6 days old cotyledonary explants showed the highest shoot regeneration frequency (61.11%). The shoot regeneration frequency was markedly enhanced by the addition of AgNO3. MS medium supplemented with 3.0 mg/L BA, 0.2 mg/L NAA and 2.0 mg/L AgNO3 showed the maximum shoot regeneration frequency (77.78%). To observe the genotypic variation for shoot regeneration potentiality 6 days old explants of six L. esculentum genotypes namely BARI Tomato 2, BARI Tomato 5, BARI Tomato 7, BARI Hybrid Tomato 4, BINA Tomato 3 and BINA Tomato 5 were cultured on MS medium supplemented with 3.0 mg/L BA, 0.2 mg/L NAA and 2.0 mg/L AgNO3. Among the six genotypes BARI Tomato 2 showed the highest shoot regeneration frequency (91.67%) and BINA Tomato 3 showed the lowest shoot regeneration frequency (44.44%). MS medium supplemented with 0.1 mg/L NAA showed the highest frequency (100%) of rooting. The regenerated plantlets were transferred in pot soil and grown to maturity.

2015-06-01T00:00:00Z RAHMAN, SYEDA KOHINOOR http://localhost:8080/xmlui/handle/123456789/355 2025-03-02T05:26:09Z 2015-06-01T00:00:00Z

GENETIC DIVERSITY OF PLANT SPECIES IN HILLY AREAS ADJACENT TO SYLHET AGRICULTURAL UNIVERSITY RAHMAN, SYEDA KOHINOOR The study was carried out to investigate the status of genetic diversity of plant species in hilly areas and plain lands of Sylhet Agricultural University (SAU) and in hilly areas adjacent to SAU during January, 2015 to June, 2015. Four study areas were selected to investigate the status of genetic diversity of plant species. It also explored relationships between the characteristics, types, habits, total number of plants and the relationships between hilly area plants and plain land area plants. Many plant species including trees, shrubs and herbs were recorded from the studied hilly areas. Several medicinal plants found in hilly areas were also recorded. Among these plant species the most prevalent tree species were horitoki, bohera, mehgoni, arjun, neem etc, and the most prevalent herb species were chanci, shaknotey, katanotey, malancha shak, salvia etc. The highest numbers of natural tree species were found in hilly areas of M. C. College adjacent to SAU whereas the lowest numbers of tree species were found in the area of Vice-chancellor’s banglaw of SAU. The highest number of shrubs and herbs were found in Vice-chancellor’s banglaw and plain land of SAU, respectively. Both naturally grown and planted trees, shrubs and herbs along with a good number of medicinal plants were found in the studied areas which exhibited the wide diversity in plant genetic resources in studied areas.

2015-06-01T00:00:00Z Tanha, Fahminaj http://localhost:8080/xmlui/handle/123456789/248 2025-01-19T10:56:35Z 2014-12-01T00:00:00Z

IN VITRO SCREENING OF BRASSICA SPECIES FOR HEAVY METAL TOLERANCE Tanha, Fahminaj The experiment was conducted at the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet, Bangladesh to find the suitable Brassica species grown in Bangladesh for tolerance of cadmium (Cd), lead (Pb) and arsenic (As), so that the appropriate cultivars can be used for genetic engineering for improving traits of heavy metal tolerance and accumulation in order to cleanup polluted agricultural soils. In this study, 5 genotypes of Brassica campestris, 3 genotypes of B. juncea and 3 genotypes of B. napus were subjected to Cd, Pb and As stresses. B. napus cv. BARI sarisha-13 was used to standardize the heavy metal tolerance (50% to 60% growth reduction) and other genotypes were used to determine their capacity of heavy metal tolerance against Cd, Pb and As stresses. Among the Brassica species the highest level of tolerance showed by B. campestris cv. BARI sarisha-9 and the lowest level of tolerance showed by B. juncea cv. Rai-5 against Cd-stress. B. juncea cv. Daulat showed the highest level of tolerance and B. campestris cv. BARI sarisha-15 showed the lowest level of tolerance against Pb-stress. Incase of As tolerance, B. juncea cv. Daulat showed the highest tolerance and B. campestris cv. Safal showed the lowest tolerance than the other genotypes of Brassica species. Therefore, two genotypes, B. campestris cv. BARI sarisha-9 and B. juncea cv. Daulat can be chosen for genetic engineering for heavy metal phytoremediation efficiently.

2014-12-01T00:00:00Z Sarker, Kishore Kumar http://localhost:8080/xmlui/handle/123456789/247 2025-01-19T10:51:33Z 2014-12-01T00:00:00Z

HIGH FREQUENCY PLANT REGENERATION OF FIELD MUSTARD (Brassica campestris) Sarker, Kishore Kumar The experiment was carried out at the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet to develop a suitable protocol for high frequency plant regeneration of field mustard (Brassica campestris). An efficient system for high frequency plant regeneration system of B. campestris was developed through investigating various factors such as combination of plant growth regulators, explant type, age and ethylene biosynthesis inhibitor AgNO3. To observe the suitable medium for callus initiation and shoot regeneration, cotyledonary and hypocotyl explants were cultured on MS medium supplemented with different concentrations of 6-Benzyl amino purine (BA) and αnaphthalene acetic acid (NAA). The ranges of callus initiation and shoot regeneration frequency of cotyledonary explants were 33.33% to 94.44% and 13.89% to 63.89%, respectively whereas hypocotyl explants showed 11.9% to 47.62% and 2.38% to 19.04% callus initiation and shoot regeneration frequency, respectively. The highest callus initiation (94.44%) and shoot regeneration (63.88%) frequency were observed on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA in case of cotyledonary explants. To observe the effect of age of explants source material on shoot regeneration 3 to 7 days old cotyledonary explants were cultured on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA and 4 days old cotyledonary explants showed the highest shoot regeneration frequency (72.22%) and higher number of shoots per explant (3.94). The shoot regeneration frequency and number of shoots per explant were markedly enhanced by the addition of AgNO3. MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L NAA and 2.0 mg/L AgNO3 showed the maximum shoot regeneration frequency (83.33%) and maximum number of shoots per explant (6.86). To observe the genotypic variation for shoot regeneration potentiality 4 days old explants of five B. campestris genotypes namely Tori-7, BARI sarisha-6, BARI sarisha-9, BARI sarisha-12 and BARI sarisha-15 were cultured on MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L NAA and 2.0 mg/L AgNO3. Among the five genotypes BARI sarisha-12 showed the highest shoot regeneration frequency (83.33%) as well as maximum number of shoots per explant (6.86) and BARI sarisha-15 showed the lowest shoot regeneration frequency (52.78%) as well as lower number of shoots per explant (3.23). MS medium supplemented with 0.2 mg/L NAA showed the highest frequency (100%) of rooting. The regenerated plantlets were transferred in pot soil and grow to maturity.

2014-12-01T00:00:00Z