Dept. of Fisheries Technology and Quality Control

COMPARATIVE STUDY ON BACTERIOLOGICAL PARAMETERS OFINDIGENOUS AND EXOTIC KOI (Anabas testudineus) AT SYLHET SADAR UPAZILA

2025-04-15T05:12:15Z

COMPARATIVE STUDY ON BACTERIOLOGICAL PARAMETERS OFINDIGENOUS AND EXOTIC KOI (Anabas testudineus) AT SYLHET SADAR UPAZILA BATEN, MD. ABDUL The present study was conducted on the bacterial population including the pathogenic bacteria in indigenous and exotic Koi (Anabas testudineus) at Sylhet Sadar Upazila during December/2014 to May/2015. For the investigation of bacterial biomass, ten (10) fish markets were selected considering the seasons winter and summer. The study concentrated on the bacteriological parameters such as Total Viable Count (TVC), Total Coliform (TC), and the prevalence of Escherichia coli, Salmonella spp. and Vibrio spp. The highest bacterial load was observed in indigenous Koi and the lowest in exotic Koi both summer and winter seasons. The highest mean bacterial load in indigenous Koi were 8.57±0.08 log cfu/g and 6.68±0.75 log cfu/g in summer and winter respectively whereas the same of exotic Koi were 8.50±0.06 log cfu/g and 6.62±0.74 log cfu/g both summer and winter. The study revealed that bacterial load in winter season complied with ICMSF standard for both types of Anabas testudineus but bacterial load of both types of same species in summer season didn’t comply with ICMSF standard. Higher temperature influenced on bacterial load because a large number of bacteria were found in this season. The observed results were statistically significant for both types of species (p<0.05) and as well as seasons (p<0.05). The highest bacterial load was observed at the highest temperature (36.3 0 C) in the month of April and the lowest was found at the lowest temperature (18 0 C) in the month of January. Temperature was positively correlated with both indigenous and exotic Koi in both the seasons. The mean Total Coliform (TC) of indigenous and exotic Koi in summer was 197 and 172, in winter 93.4 and 87.1 MPN/g. Total Coliform in winter season complied with ICMSF standard but TC in summer didn’t comply with this standard. The highest TC was found in indigenous Koi (290 MPN/g) in summer season whereas the lowest (34 MPN/g) was in exotic in winter season. The isolated pathogenic bacteria were Escherichia coli, Salmonella spp., and Vibrio spp. In indigenous koi the percentage of these isolated bacteria were 90%, 80% and 70%, respectively whereas the same in exotic Koi were 80%, 70% and 50% in summer season. Similarly, in case of indigenous these were recorded 80%, 60%, 50% and the same in exotic Koi were 70%, 50% and 60% in winter season. This findings of this study indicated that the prevalence of all types of observed bacteria were lower in exotic Koi than indigenous in summer and almost the same results in winter. The studied varieties of Anabas testudineus (indigenous and exotic) were more or less contaminated but comparatively higher bacterial load and pathogenic bacteria in indigenous Koi than exotic Koi both summer and winter season. The results of this study indicated that the TVC, TC and all other identified pathogenic bacteria were higher in indigenous Koi both summer and winter than exotic of the both seasons. The result which don’t comply the ICMSF standard is alarming for food safety as well as not considered for export.

EFFECT OF GILL REMOVAL AND GUTTING ON THE QUALITY OF FISH UNDER DIFFERENT STORAGE CONDITION

2025-03-19T04:16:53Z

EFFECT OF GILL REMOVAL AND GUTTING ON THE QUALITY OF FISH UNDER DIFFERENT STORAGE CONDITION UDDIN, WAYEZ The study was conducted to investigate the effect of gill removal and gutting on the quality of fish during different storage period. Fish samples were collected from a local market of Sylhet. Quality changes in fish during different storage condition were investigated by using organoleptic, biochemical and microbiological aspects. There were two groups of samples of which one were intact and other was gill and gut removed. During the storage period, the processed samples showed an extended shelf life at all storage conditions with higher nutritional value. The moisture content in intact sample was ranged from 77.82% to 76.13%, 77.82% to 80.07%, 77.82% to 68.11% and in case of processed sample 77.42% to 75.89%, 77.82% to 78.57%, 77.82% to 67.11% at ambient temperature, ice condition and frozen condition respectively. The protein content in intact sample was ranged from 16.91% to 15.16%, 16.91% to 13.43%, and 16.91% to 13.03% and in case of processed sample 16.91% to 15.56%, 15.09% to 14.20% at ambient temperature, ice condition and frozen condition respectively. The lipid content showed an inverse relation with moisture content and ranged from 3.21% to 3.17%, 3.21% to 1.97%, 3.21% to 3.27% in case of intact sample and 3.21% to 3.13%, 3.21% to 2.05%, 3.21% to 3.40% in case of processed sample in different storage period. The ash content of intact sample was ranged from 2.11% to 2.41%, 2.11% to 1.23%, and 2.11% to 2.64% whereas the result of the processed sample was 2.11% to 3.9%, 2.11% to 1.57% and 2.11% to 2.79% at different periods of ambient temperature, ice condition and frozen condition respectively. The pH value of the intact sample was ranged from 7.10 to 8.32, 7.10 to 6.67, 7.10 to 8.25 but the pH value of processed sample was ranged from 7.10 to 7.99, 7.10 to 6.99 and 8.39 at different periods of ambient temperature, ice condition and frozen condition respectively. Microbial load of the intact sample increased from 3.11×10 CFU/gm in ice stored sample whereas microbial load of processed sample was 2.7×10 5 CFU/gm to 8.5×10 8 day of observation. On the other hand, microbial load of the Sample A at frozen storage decreased from 3.11×10 4 CFU/gm to 5.2×10 6 CFU/gm at 15 th CFU/gm but the microbial load of the processed sample decreased at a level of 2.7×10 5 CFU/gm to 4.8×10 4 CFU/gm. Finally it is revealed that the removal of gill and gut decrease the rate of spoilage in fish which resulted in an extension of shelf life.

Analysis of Bacterial Load and Pesticide Residues in Dried Punti Fishes Collected from Different Sources of Sylhet Region

2025-03-19T04:09:26Z

Analysis of Bacterial Load and Pesticide Residues in Dried Punti Fishes Collected from Different Sources of Sylhet Region KABIR, MD. LUTFUL The present study was conducted to analyses bacterial load and pesticide residues in dried punti fishes from three different sources like drying yards, market and raw fish from the same sources of drying yards of Bishwanath and Sadar upazilla of Sylhet district during December 2014 to April 2015. For microbiological analysis total plate count (TPC) method was used to estimate the bacterial load in the laboratory of the Department of Microbiology and Immunology, Faculty of Veterinary and Animal Science, SAU. Mean bacterial loads of drying yard, market and control sample were estimated 5.63±1.29×10 6 , 7.76±2.07×10 6 and 1.66±0.66×10 6 cfu/g, respectively. According to the International Commission on Microbiological Specification for Food (ICMSF) its quality was found in the marginally accepted category (5×10 5 7 - 10 cfu/g). Higher bacterial load indicated lack of hygiene and sanitation during dried fish processing. A total of 27 dried Punti fish samples of three different sources were taken for the quantification of pesticides in the Pesticide Analytical Laboratory, Division of Entomology, BARI, Gazipur. The modified QuEChERS method was used to extract pesticides from dried Punti fish matrices and GC-ECD method was used for the determination of organochlorine pesticide residues. Around 22% samples were observed contaminated with three organochlorine pesticides i.e. Aldrin, Dieldrin and Endrin either single or multiple product residues. Among the contaminated samples 22.22% were of drying yard and 44.44% from market. Aldrin was found in all six contaminated dried punti fish sample. Higher residue level was found in multiple product containing samples with Aldrin in comparison to Endrin and Dieldrin. All of the detected levels (0.332-0.967 ppm) were above MRL (0.1ppm) irrespective of single or multiple pesticide residues. The presence of multiple pesticides in the samples indicates that the owners of the drying yards as well as retailer/venders are applying more than one pesticide in the dried punti fishes. Although Aldrin, Dieldrin and Endrin are already banned in Agriculture but their presence is still remaining. These higher concentration levels of organochlorine pesticides in the dry punti fishes might cause health hazard to the consumer which is very alarming for food safety issue.

STUDY ON BACTERIOLOGICAL QUALITY OF FROZEN AND UNFROZEN PABDA (Ompok pabda) IN THE FISH PROCESSING PLANT

2025-03-19T03:55:54Z

STUDY ON BACTERIOLOGICAL QUALITY OF FROZEN AND UNFROZEN PABDA (Ompok pabda) IN THE FISH PROCESSING PLANT MAJUMDER, PROTIK KUMAR The present study was investigated to determine the bacteriological quality of frozen and unfrozen Pabda (Ompok pabda) in the Fish Processing Plant (Eurocross Fish Processing Plant, Khadimnagar, Sylhet) during the period of January/2015 to October/2015. For this experiment five (5) frozen samples from five different lots and five (5) unfrozen samples which were ready for the processing and also preparation for the respective lots were taken for the estimation of bacteriological quality of fishes. The study was considered the bacteriological parameters such as Total Viable Count (TVC), Total Coliform (TC), and the occurrence of Escherichia coli, Salmonella spp. and Vibrio cholera. The TVC of frozen samples were 2.9×10 5 5 5 , 1.8×10 5 , 1.5×10 , 2.5×10 and 5 3.5×10 CFU/g and furthermore. On the other hand, the TVC of unfrozen samples were 5×10 7 6 5 , 3×10 , 6×10 , 6 7 4×10 and 4×10 CFU/g respectively. The mean bacterial load of Frozen and Unfrozen Pabda were log 5.37±0.15 and log 7.02±0.59. The frozen samples contained lower bacterial load than unfrozen samples. The bacterial loads of frozen Pabda comply with ICMSF standard but the same of unfrozen Pabda does not comply with this standard. The TC of frozen samples were 20, 15, 20, 21 and 27 MPN/g and furthermore, whereas the TC of unfrozen samples were 160, 120, 120, 120 and 150 MPN/g respectively. The mean Total Coliform for both frozen and unfrozen samples were 20.6±4.28 and 134±19.49. From this study, it is very revealed that the Total Coliform of frozen pabda complies with ICMSF standard but the same of unfrozen Pabda does not comply with this standard. The detected pathogenic bacteria were Esherichia coli, Salmonella spp. and Vibrio cholera. In frozen Pabda all of the identified pathogenic bacteria were absent. All of the unfrozen samples were contaminated with Esherichia coli, but two of the samples were contaminated with Salmonella spp. and one sample polluted with Vibrio cholerae. The results of this study indicated that the TVC, TC and all other identified pathogenic bacteria were higher in unfrozen Pabda than frozen Pabda. So, the bacteriological quality of frozen Pabda complies with ICMSF standard but the same of unfrozen samples does not comply with ICMSF standard. The results are more important for ensuring food safety as well as for export.