ISOLATION, ID ENTIFICATION AND ANTIBIOGRAM STUDY OF ESCHERICHIA COLI FROM NATIVE CHICKEN AT SYLHET REGION

Abstract

The study was conducted to isolate and identify Escherichia coli occurring naturally in healthy chickens. The study was also focused on the investigation of drug sensitivity and resistance pattern of the isolates. A total number of 50 field samples (cloacal swab) were collected from apparently healthy native chickens from different areas of the Sylhet region. 10 samples from Bandar Bazar, 10 samples from Ambarkhana Bazar, 10 samples from Eidgah Bazar, 10 samples from Baluchor Bazar and 10 samples from University residencial area. Out of these 50 samples of apparently healthy chicken the number of samples found to be positive for Escherichia coli were 4 of Bandar Bazar samples , 4 of Ambarkhana Bazar samples , 3 of Eidgah Bazar samples , 3 of University residencial area samples and 2 of Baluchor Bazar samples . This study reveals that the prevalence rate is 40% of Bandar Bazar , 40% of Ambarkhana Bazar ,30% of Eidgah Bazar , 30% of University residencial area and 20% of Baluchor Bazar . All of the Escherichia coli isolates revealed the same morphological ,cultural and biochemical characteristics. The antibiotic sensitivity pattern showed that among the Escherichia coli isolates (16 samples) highly sensitive to ceftriaxone, intermediate sensitive to nalidixic acid, cephalexin, gentamycin, erythromycin and chloramphenicle and fully resistant to penicillin and bacitracin. It may be concluded that the high resistance of Escherichia coli to antibiotic may constitutes a threat to public health in Bangladesh.