| dc.description.abstract | Salmonellosis is a leading cause of food borne illness worldwide with an estimated millions cases each year in the
world. Transmission of this organism occur through horizontally and vertically. The source of the infection is
usually difficult to determine, although carrier animals introduced into the flock are considered the most common
source. A point of time study was conducted from July 2015 to November 2015 to isolate and identify the
Salmonella spp from the broiler chicken and calculate the prevalence among the broiler birds in Sylhet sadar. The
commercial broiler farms are located in various locations of the Sylhet sadar and 4 major commercial broiler
markets of Sylhet sadar were taken into the count for the collection of the sample. Different chemicals and
reagents were used for conducting the biochemical tests: Grams stain, Methyl red solution, Methyl-red VogesProskauer
(MR-VP) broth, Kovacs reagent, Potassium hydroxide solution, Phosphate buffered saline (PBS)
solution, phenol red Dextrose, Maltose, Lactose, Mannitol and Sucrose. Other chemicals used for the experiment
were 20% glycerin, liquid paraffin, wax, Alcohol, spirit etc. The isolates of Salmonella spp. showed positive result
in Catalase test. In Grams staining under microscope, the Salmonella revealed Gram-negative, pink color, small
rod shaped and arranged in single or paired. Motility test was fundamental basis for the detection of motile and
non motile Salmonella organisms. The prevalence of salmonellosis at different farm level were 38.89%, 30.76%,
16.67%, 37.5%, 20.0%, 20.0%, 22.22%, 45.45% respectively. The overall farm level prevalence was obtained
31.25%. The prevalence in different commercial broiler markets were 66.67%, 64.28%, 37.50%, 33.33%
respectively. The overall prevalence in commercial broiler markets was 55%.Age wise prevalence found 14.28%
for 0-10 days, 40.62% for 11-20 days and 45.45% for 21 to above day’s birds. Retail chicken seller should be
conscious about the problems of overcrowding and unhygienic management. They should be aware of proper
sanitary management, handling transportation and litter management of broiler chicken in market. Feed should be
supplied in hygienic condition. | en_US |
