EFFECT OF CONCENTRATE FEEDS ON METHANE EMISSION IN BEEF CATTLE BY USING IN VITROTECHNIQUE

Abstract

This experiment was conducted to investigateeffect of concentrate feeds on methane emission in beef cattle by using in vitro technique using rumen fluid from beef cattle and to compare in vitro methane production using different concentrate feeds. There were 7 concentrate feeds stuffs such as Rice Polish, Wheat bran, Mustard oil cake, Broken rice, Green gram, Grass pea and Red lentil were collected from local market of Sylhet, Bangladesh. Three indigenous beef cattle (Bosindicus) were selected for this experiment. Rumen fluid from 3 beef cattle was collected immediately after slaughtered. All the seven concentrate feeds were used to study their effects on gas production, methane production and IVDMD for 3, 6, 12, 24 and 48 hours. 0.3 g grindedfeed sample was separately weighted by electric balance and taken into 21 syringes. Then they were placed on a stand and put into the water bath by maintaining the temperature 39±.5°C. According to the back change of the piston position gas production was measured at 24 and 48 hr of incubation. CF, EE and Ash were higher in low quality concentrate while DM, OM, CP and IVDMD were higher in high quality concentrate. Among the low quality concentrate total gas production rate was highest in wheat bran at 6, 12, 24 and 48 hr of incubation and total gas production rate was lowest in mustard oil cake; on the other hand within high quality concentrates total gas production was lowest in grass pea and highest in green gramat 6, 12, 24 and 48 hr of incubation. Among the low quality concentrates amount of methane production was highest in wheat bran and lowest in mustard oil cake at 24 and 48 hr of incubation period. Among the high quality concentrate methane production rate was highest in green gram and lowest in grass pea at 24 hr of incubation. Among the high quality concentrates methane production rate was lowest in grass pea and highest in green pea at 48 hr of incubation. In this study it was observed that CF, NFE and CP had positive, moderate and negative effects on methane emission respectively.